answer 1:2 - Hill plot
The Hill plot does not yield a statistically sound analysis because it overmphasizes the experimental error at the extremes of the plot and minimizes it at the center; however, if only the center of the plot is used for linearization, it may give useful indications for sucessive analysis using sounder methods. It the present case the Hill plot yields a slope (Hill coefficient n) of approximately 0.8. A Hill coefficient lower than unity is characteristic of a chemically heterogeneous sample or of a negatively cooperative oligomeric protein.
We have three possible hypotheses:
1) The protein is not a monomer and presents negative cooperativity.
2) The protein is not a monomer and its subunits are functionally non-equivalent.
3) The protein is a mixture of different monomeric isoforms.
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