answer 3:1 - least squares fit of Y = [X]/(K+[X])
The use of least squares minimization is the soundest statistical way of analyzing the experimental data. You use the following equation to calculate the fraction of liganded sites:
The equilibrium constant obtained is Kd = 1 mM (for the dissociation reaction: PX <==> P + X).
Do the fit and the residuals look good?
Back one step